Florida biotech firm Hesperos, Inc., in collaboration with pharmaceutical giant Roche and the University of Central Florida (UCF), has shown that one of its innovative multi-organ in vitro (out of body) model systems is able to realistically replicate in vivo (in body) responses to anticancer therapies for both the parent drugs and their metabolites to determine therapeutic index for both single drugs and drug-drug combinations.
The therapeutic index measures relative safety of a drug and the range in which a drug dose is determined to have a therapeutic effect before significant toxicity begins to occur. The initial determination of efficacy at the same time can currently only be done at the preclinical stage in animals, and animal models are not always a good guide to how a drug will perform in humans.
"This is a game changer in the preclinical drug development process, which normally requires an animal model to measure therapeutic index, and in the case of many rare diseases requires testing in humans as there are no animal models available," said Hesperos Chief Scientist James J. Hickman, who is a Professor at UCF's NanoScience Technology Center. "In addition, our system will allow testing of different therapies on small samples of a specific cancer patient's tissue to help inform doctors about which treatment works best for each individual."
"With this system, medicinal chemists can test multiple variations of a drug candidate with milligram quantities of the compound, at the pre-animal stage. Normally to go into animals, scale-up needs to occur to manufacture grams to kilograms of a compound, which generally limits animal trials to one candidate because it is expensive," stated CEO and President Michael Shuler, also Professor Emeritus at Cornell University.
As reported in the prestigious peer-reviewed scientific journal, the Hesperos team tested their device in two scenarios: on cancer-derived human bone marrow cell lines for anti-leukemia drug analysis, and on vulva and breast cancer cell lines to test multi-drug treatments in a multi-drug resistant cancer.
For both configurations, a body-on-a-chip system was developed with the capacity to house multiple human-derived organ-like tissue constructs grown on an array of biological microelectromechanical systems (bio-MEMS) modules in a single recirculating serum-free medium that allows non-invasive measurements of responses in the human surrogate.
For the leukemia model, two bone marrow components were incorporated with liver tissue to measure the cytostatic effects of two anticancer drugs - diclofenac and imatinib - on bone marrow-derived cells and off-target effects on the liver. Testing showed that liver viability was not affected by imatinib, but was reduced by 30% with diclofenac in the first configuration.
In the second configuration, one multi-drug resistant vulva cancer cell line and one breast cancer cell line without multi-drug resistance were incorporated into the system with a liver compartment to determine metabolic effects, and with functional cardiac models to measure electrical and mechanical deficits from off-target toxicity. The common breast cancer drug tamoxifen reduced viability of the breast cancer cells only after being processed by the liver. Tamoxifen did not affect the vulva cancer cells except when co-administered with verapamil, a permeability-glycoprotein (Pgp) inhibitor. Both tamoxifen alone and co-administration with verapamil produced off-target cardiac effects, as indicated by a reduction of contractile force, beat frequency, and conduction velocity, but did not affect viability.
These results were consistent with what has been reported in human trials. But they were done in a lab, without the need of animal studies and with no risk to humans.
Hesperos, Inc. is the first company spun out from the Tissue Chip Program at NCATS (National Center for Advancing Translational Sciences), which was established in 2011 to address the long timelines, steep costs and high failure rates associated with the drug development process. Hesperos currently is funded through NCATS' Small Business Innovation Research program to undertake these studies and make tissue chips technology available as a service based company.
"One of the many uses of tissue chip technology is to make clinically relevant assessments of the effectiveness of candidate drugs," said Danilo Tagle, Ph.D., associate director for special initiatives at NCATS.
The ability of these systems to assess cardiac function non-invasively and monitor biomarkers over time, using multiplexed and repeat drug dosage regimes, provides an opportunity to run long-term studies for chronic administration of cancer drugs or drug-drug combinations.
Christopher W McAleer, Christopher J Long, Daniel Elbrecht, Trevor Sasserath, L Richard Bridges, John W Rumsey, Candace Martin, Mark Schnepper, Ying Wang, Franz Schuler, Adrian B Roth, Christoph Funk, Michael L Shuler, James J Hickman.
Multi-organ system for the evaluation of efficacy and off-target toxicity of anticancer therapeutics.
Science Translational Medicine 19 Jun 2019: Vol. 11, Issue 497. doi: 10.1126/scitranslmed.aav1386.